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The substrate Answer was then stored at −20°C right until the ammonium and nitrate contents ended up analyzed Using the San++ Constant-Movement Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate perseverance was according to a reaction with hydrazinium sulfate, sulfanilamide and N-(1-naphthyl) ethylenediamine dihydrochloride which res